Tyrosylation and purification of peptides for radioiodination
Summary
Type: HPLC Application #: 108130
Catalog#: 720065.40 - MN HPLC Column, Nucleosil C18, 300A, 5um, 4x250mm
Detection: UV, 230nm Conditions: eluent: 0.1% TFA in water with a gradient of acetonitrile as indicated (dotted line) flow rate 1 ml/min Authors: Gether U Source: J Chromatogr 447 (1988) 341 - 349
Details
Legend: Fig. 1. Derivatization of pancreatic polypeptide (a 36-amino acid, relatively
non-polar peptide) and hydrolysis of the derivatization reagent; upper panel:
HPLC profile after 0.5min incubation, lowe